Showing posts with label solid. Show all posts
Showing posts with label solid. Show all posts

Friday, August 30, 2013

solid wood Chess-board

005.JPGI love playing Chess. Especially in the summer, sitting outside in the shade with a good friend and a cool beverage.
I have gone thru several boards thru-out the years and I have always wanted a good solid wood board. When the Toy contest opened and I saw the Maker-bot as a prize, I knew exactly what I wanted to enter.

In this instructable I am taking you through the steps of making a Chess-board.
The wood used for the squares are Cherry and Norway Maple, the border is made from Norway maple. All of the wood used was harvested from local tress fallen during storms. After a really bad storm I ALWAYS drive around with a chainsaw offering to help and haul away some of their larger pieces (so glad I have a trailer).

The actual squares are 1-1/2" X 1-1/2". The whole board with the border is about 12-1/2" X12-1/2".

chess 002.JPGI'm really not one to draw up and go by plans. I really don't even like following plans. That being said, I do sketch ideas and make notes to figure out my rough needs for a project. This does have draw-backs at times, but I'm still able to "wing-it" with most projects.

A Chess/Checker-board consists of 64 squares total (8 columns and 8 rows).

After a quick sketch and some math, I found a couple of boards. The boards I have are rough sawn 5/4 boards (VERY ROUGH).


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Sunday, June 23, 2013

How to make solid culture medium plates for yeast culture

IMG_4024.JPGIMG_4052.JPGYou will need several 100 mm borosillicate glass pyrex (lab-grade) petri dishes which can be purchased from Amazon, along with lab-grade pyrex Erlenmeyer flasks of appropriate size for the quantity of plates that you want to make.  Here I am using two pyrex petri dishes and two small Erlenmeyer flasks, since I am only making two plates and a small slant (50 mL).  If you want to also make slants, then you should purchase borosillicate glass 'slant tubes' with screw top.  Also, some other microbiological instruments like a spreader and inoculation loop could be helpful.  I made it at TechShop with some scrap metal, pliers, and a vise.  www.techshop.ws 

You will also need the raw materials that will make the solid culture medium.  You can obtain laboratory-grade materials from Amazon, or you can make your own 1.040 wort and use yeast nutrient and agar-agar to solidify the plates.  You can buy D-(+)-Maltose, Peptone, yeast extract, and agar technical (solidifying agent) on Amazon, believe it or not (autoclave the maltose separately to prevent caramelization of the sugar).  While this method produces solid growth medium that is perfect for keeping yeast in a laboratory setting, many folks don't want to shell out the dough for these materials, and prefer to make use of beer-making equipment and materials they might have at home already.  

The easy way to do this is to get agar-agar (solidifying agent similar to gelatin) online or at an Asian food store.  Then, make a simple 1.040 gravity wort using only brewer's 2-row base malt, add yeast nutrient and 20 grams of agar-agar into 1 liter of culture liquid (can be scaled down accordingly depending on your needs), and autoclave in an appropriately-sized Erlenmeyer flask (you don't want to exceed about 50% of the volume of the flask when autoclaving).  Personally, I don't have a pressure cooker capable of autoclaving my 2 liter Erlenmeyers upright and full of liquid, so I make small batches.  You can easily make up 1.040 wort and split into several batches (in mason jars) for autoclaving purposes, and these will keep in the fridge or basement as long as they are sealed.  Then, you can use this liquid for starters or 50-100 mL at a time for making solid culture medium plates such as we are discussing.  In this case, you would take 50 mL of this nice pre-autoclaved wort and add 1 gram of agar-agar to it, dissolve, and autoclave again in an Erlenmeyer flask in your pressure cooker.  


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